Grape seed oil with acid value of 15.12±0.016 mg KOH/g, was used as raw material, and the effects of chemical, physical and enzymatic deacidification on the content and kind of trans fatty acids in the grape seed oil were investigated. The changes of trans fatty acids content and acid value in different refined technologies were discussed. The acid values were reduced to 0.10±0.022 mg KOH/g, while the content of trans fatty acids increased by 0.31±0.0011% when chemical refined was employed. The optimal conditions of physical deacidification of the grape seed oil were deacidification temperature 240 ℃and time 8 h, under which the acid value of 0.13±0.018 mg KOH/g, and trans fatty acids content of 1.86% were obtained. The conditions of enzymatic esterification to deacidificate the grape seed oil were as follows: temperature 80 ℃, addition of glycerol 300% and enzyme 3% for 8 h. Under these conditions, the acid value declined to 2.36±0.026 mg KOH/g, while the content of trans fatty acids increased from 0.034±0.001% to 0.035±0.001%~0.041±0.0016%. Among the methods tested, the enzymatic esterification deacidification has the least influence on trans fatty acids content and followed by chemical refining.
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