Antioxidant Activity and Molecular Weight Distribution of Hydrolysates from Paphia Undulate Protein
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Abstract:
Three types of protein including sarcoplasmic protein, myofibrillar protein and stromal protein from Paphia undulate were isolated. The amino acid composition, antioxidant activity in vitro and molecular weight distribution of the hydrolysates prepared with trypsin were investigated. All of the three protein fractions contained higher contents of essential amino acids, alkaline, hydrophobic and branched-chain amino acid. Protein hydrolysates showed a good radical scavenging activity with dose-effect. The hydrolysates of sarcoplasmic protein with the highest contents of small molecule peptide exhibited the strongest radical scavenging activities including DPPH radical, hydroxyl radical and superoxide anion radical, with the IC50 values being 4.82, 4.70 and 3.75 mg/mL, respectively and the molecular weight less than 1500 u accounted for 42.55±0.27%. The hydrolysates of sarcoplasmic protein and myofibrillar protein showed similar reducing power and their OD700 were 0.58 and 0.57 at 10 mg/mL, respectively. While the hydrolysates of stromal protein displayed lower reducing power.
