Establishment of the Cell Suspension Culture System of Cyclocarya paliurus and Matrix Consumption Laws
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Cyclocarya paliurus is a unique plant resource in China. As one of the threatened plants, it has been received increasing attention. Cyclocarya paliurus is rich in flavonoids, polysaccharides, triterpenoids and other bioactive substances. However, the its application is restricted for the problems of its sexual and asexual reproduction. Nowadays, employing cell culture technology to get more bioactive compounds is a vital way to make full use of Cyclocarya paliurus. The suspension cell system influenced by the factors of inoculum density, the liquid medium volume per flask, pH, sucrose concentration, phytohormone combinations and the consumption rules for culture medium in the process of cells proliferation were studied. The dynamic curve of cells proliferation and total flavonoids, polysaccharides and triterpenoids synthesized in the suspension cell system were measured. The result revealed that the preferable conditions for suspension cells proliferation were as follows: MS medium supplemented with 1.0 mg/L KT, 0.1 mg/L 2,4-D, 0.5 mg/L NAA and sucrose 30 g/L. The optimum culture situations were seed volume 8%, liquid medium volume per flask 40% and initial pH 5. Moreover, the proliferation of Cyclocarya paliurus cells correlated closely with the consumption of sugar, nitrate and phosphate. After 8 days, sugar and phosphate in the medium were absorbed completely, while NH4+ was utilized faster than NO3-, and the concentration of NH4+ showed a linear decreasing trend.
