Abstract:Polysaccharides (PUG-1) were prepared from Paphia undulate by protease hydrolysis, and the effect of PUG-1 on blood lipid of hyperlipidemia model mice was investigated. PUG-1 was separated to two fractions named PUG-1a and PUG-1bby DEAE-52-cellulose exchange chromatography, with relative molecular weight of 30.0 kDa and 10.0 kDa respectively. The glycosaminoglycan contents of PUG-1a and PUG-1b were 68.3% and 62.8%, respectively. And the ratio of gucosamine, galactosamine, hexuronic acid and sulfate in PUG-1a and PUG-1b were 1.8:0.9:1.3:1.0 and 1.5:0.8:0.9:1.0, respectively. FT-IR spectrum showed the characteristic absorption of glycosaminoglycan. The hyperlipidemia model mice were established by feeding with high fat diet (basal laboratory diet with 2% cholesterol). Meanwhile, the mice were administered PUG-1 orally at dosage of 400, 200 and 100 mg/kg?d, respectively. Normal control and positive control group were treated with distilled water and Simvastatin (0.2 mg/kg?d). The contents o f total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-c) and high density lipoprotein-cholesterol (HDL-c) in serum were examined after four weeks. The PUG-1 decreased the contents of TC, TG and LDL-c in serum and increased HDL-c significantly (p<0.05 or p<0.01) in the experimental hyperlipidemic mice. These results suggest that PUG-1 can modulate the level of blood lipid in hyperlipidemia mice and can be prevention of atherosclerosis.
