Purification of Flavonoids from Potentilla fulgens and Identification by HPLC-ESI-MS/MS
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Abstract:
Purification effects of seven kinds of macroporous resins through static adsorption on the flavonoids from Potentilla fulgens were studied. The factors including the flow rate, pH of the sample solution, ethanol concentration and pH of the desorbent were determined through dynamic adsorption. The results showed that macroporous resin D101 was the best to purify flavonoids from Potentilla fulgens. The optimal purifying conditions were as follows: the adsorption flow rate 2 BV/h, sample solution pH 4, desorbent pH 8, and 60% ethanol in the desorbent and the purity of flavonoids increased from 28.24% to 46.60%. The flavonoids were qualitatively identified using high performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS/MS), according to retention time, molecular ions, and fragment ions in MS/MS. Favonoids quantitative analyzed by external and internal standard methods through multiple reactions monitoring (MRM) to monitor specific fragment ions and standard curves of three standard substances. The main components in the flavonoids and their contents were apigenin-7-O-β-D-glucuronide (6.66 μg/mg), hyperoside (2.39 μg/mg), astragalin (2.30 μg/mg), scutellarin (1.95 μg/mg), luteoloside (0.44 μg/mg), potengriffioside A (0.39 μg/mg), and isovitexin (0.01 μg/mg).
