Resonance Light Scattering Method for Determination of Mercury Content in Fish
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Abstract:
The fish samples were pretreated by using microwave digestion Based on the obvious enhancing effect of Hg2+ in acetic acid- sodium acetate buffer solution on resonance light scattering (RLS) produced by 1,10-phenanthroline monohydrate and brilliant yellow, RLS method for determination of trace amount of mercury in fish was established. The results showed that the optimal determination conditions were: 1.5 mL acetic acid-sodium acetate buffer solution at pH 5.7, 1.0 mL 1.0×10-4 mol/L 1,10-phenanthroline monohydrate solution and 0.8 mL 4.0×10-5 mol/L brilliant yellow, reaction temperature 25 ℃ and reaction time 5 min. Good linear relationship was obtained between the enhanced RLS intensity and Hg2+ concentration in the range of 0~50 μg/L with the maximum RLS peak at 451.6 nm. The detection limit was 0.54 μg/L. Interfering ions as Cu2+ and Fe3+ could be masked by a mixture of sodium fluoride and thiourea. The result of measured mercury content in fish was in accordance with that measured by cold vapor atomic absorption spectrometry. The relative standard deviation was 1.38~2.19% (n = 5), and the average recovery was 99.33~102.00%. This method was sensitive, accurate, simple, rapid and environmentally friendly, which was suitable for determination of mercury in fish.
