Effect of Different Wavelength Ultraviolet Radiation on Photoreactivation in Vibrio parahaemolyticus
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Abstract:
The low pressure mercury lamp is used as a common tool for disinfection, with wavelength 254 nm as light source. Previous results suggested that when connected with light-emitting diodes (LED), UVA could kill bacterial effectively. In this research, we found that the disinfection effect of UVA/LED was much stronger than that of UVC; meanwhile, the regeneration of Vibrio parahaemolyticus after radiated by UVA/LED was more slowly than that only exposed by UVC. The results showed that photoreactivation did not happen in Vibrio parahaemolyticus after radiation by UVA/LED, and gene expression of photoreactivation related enzymes was not induced either. However, after UVC radiation, the photoreactivation happened and the gene expression level of photolyase increased. Thus results indicated that UVA/LED might inhibit photoreactivation in vibrio paraheamolyticus. Nevertheless, UVA/LED equipment needs much higher energy than UVC disinfection device, which should be improved.
