Effects of Gynura procumbens on the Blood Glucose and Lipid of Mice
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Hyperglycemia and hyperlipidemia have become strong threat on human health. This study investigated hypoglycemia and hypolipidemia effects of Gynura procumbens on mice, and to elucidate its mechanism by molecular biology methods. Forty Kunming mice were randomly divided into 4 groups: control, 2%, 4% and 8% Gynura procumbens fed for 12 weeks. Body and organs were weighed, and serum biochemical index and lipids levels in the liver and the feces were measured. The changes of mRNA and protein expression levels were analyzed by qRT-PCR and western blotting, respectively. Gynura procumbens had no significantly influence on body and organs weight in mice. Concentrations of triglycerides (TG) in blood, total cholesterols, low density lipoprotein (LDL) cholesterols, glucose in serum, and the hepatic TG level were remarkably decreased by feeding 4% GPM compared with control, while feces TG level obviously increased. The enzyme activity, mRNA and protein expression levels of HMG-CoA reductase (HMGCR) were significantly reduced by feeding 8% GPM, while mRNA and protein expression of Glut4 were up-regulated considerably by Gynura procumbens. The results suggested that Gynura procumbens had hypoglycemic and hypolipidemic efforts through regulating protein and mRNA expression of HMGCR and Glut4, and inhibiting TG absorption in intestinal tract.
