Water-holding Capacity and Water Content of Myofibrillar Protein Gel by Low-field Nuclear Magnetic Resonance
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Abstract:
Low-field nuclear magnetic resonance (NMR) and traditional drying method were analyzed to determine water holding capacity (WHC) and water content of myofibrillar protein (MP) gel. The results showed that determination of the spin-spin relaxation time (T2) of MP gel could reflect the compositions and distributions of the three states of water (bound water, immobile water and free water) in MP gel. The higher the free water percentage, the lower the WHC and the higher the water content. The proton density images of MP gels were obtained by magnetic resonance imaging (MRI), which could reflect the content and spatial distribution of water in MP gels. The higher the grey value of proton density image, the higher the water content of MP gel. Pearson’s correlation analysis showed that the grey value, the contents of immobile and free water that measured by low-field NMR were significantly correlated with the results that measured by the traditional drying methods (p<0.05), and the correlation coefficient were 0.97, 0.96 and 0.96, respectively. In conclusion, the low-field NMR technique can be used to determine WHC and water content of MP Gel.