A self-developed gutted mechanism were employed for cutting Trachurus japonicus and Synodus macrops to avoid fish damage and increase gut removal rate in gutted process. The relationships of acceptability evaluation with transmission speed, cut knife rotation speed and gutted wheel rotation speed were studied by quadratic regression rotational combination design, and the mathematical model between the acceptability evaluation and operating parameters was established. The best parameters of gutted process of Trachurus japonicus were: transmission speed 1.92 m/s, cut knife rotation speed 1154.0 r/min,and gutted wheel rotation speed 60.0 r/min. And the optimized parameters for Synodus macrops were: transmission speed 1.39 m/s, cut knife rotation speed 1280.0 r/min, and gutted wheel rotation speed 95.0 r/min. Under these conditions the maximum value for acceptability evaluation of gutted effect was achieved.