Screening and Identification of a Xylanase-producing Strain during Tobacco Fermentation
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Abstract:
Microorganisms play an important role in tobacco aroma improving. The tobacco samples were selected from five areas (Bijie, Chuxiong, Kunming, Qujing, and Xiangxian) of three years (2007, 2008 and 2009), using nutrient agar for purification, and then cultured in the screening xylanase base for rescreening. The xylanase-producing strains was screened by determining the appear transparent circles among the colonies after Congo red staining. The xylanase activities of these strains were measured by 3,5-Dinitrosalicylicacid (DNS). Qb4 strain, which had higher xylanase activity, were identified as Bacillus methylotrophilus by analysis of its morphological, physiological and biochemical characteristics, carbon source utilization and the 16S rRNA conserved sequence.
