Determination of 10 Pesticide residues in Fruits by HPLC with SPE and UV Double Wavelength
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Abstract:
HPLC with solid-phase extraction (SPE) and UV double wavelength was presented to analysis of imidacloprid, acetamiprid, carbendazim, thiophanate-methyl, primigravida, diflubenzuron, chlorbenzuron，phoxim, difenoconazole and abamectin pesticide residues in strawberry, peach and apple. Sample preparation involves extraction with acetonitrile, and then purification and enrichment by LC-NH2 solid. Chromatographic analysis was performed on Shiseido CAPCELL PAK MG ⅡC18（250 mm×4.6 mm, 5 μm）HPLC column using methyl alcohol, water and acetonitrile as gradient eluents. Dual wavelength at 270 nm and 245 nm was conducted to detect target compounds. All the ten pesticides ranging from 0.05 mg/kg to 1.0 mg/kg had a good linear relationship (0.9834~0.9992). And the limits of detection and quantification for the ten pesticides were 0.005~0.030 mg/kg and 0.020~0.098 mg/kg, respectively. The average recoveries obtained from samples spiked with standards at concentrations of 0.05, 0.10 and 0.20 mg/kg were in the range of 69.82~88.95%，86.77~110.61% and 89.74~110.62%, respectively, with relative standard deviations (RSD) of 6.52~13.24%, 5.21~10.32% and 3.56~9.88%, respectively. This method can be applied to determine and quantitative analysis of 10 pesticide residues in fruits, which was more convenient operation, higher sensitivity, higher accuracy and better repeatability than other methods.
