Determination of Organic Acids in Fermentation-silage of Citrus Peel Wastes by RP-HPLC
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Abstract:
Reversed phase high performance liquid chromatography (RP-HPLC) was used to detect six different organic acids from fermentation-silage of citrus peel wastes. The organic acids were extracted by heated water from samples and analyzed by RP-HPLC on an applied Welch Materials XB-C18 column (4.6 mm×250 mm, 5 μm) at 214 nm using the mixture of 0.05 mol/L monopotassium phosphate (pH 2.70) and methanol (97:3, V/V) as mobile phase at a flow rate of 0.80 mL/min. The injection load was 20μL and the column temperature was 20 ℃. Under these conditions, the six organic acids had a good separation within 20 minutes and the recovery rates ranged from 94.96 % to 103.43%. Each organic acid presented a good linear relationship between concentrations and chromatographic peak area with the correlation coefficient higher than 0.99 (R2 > 0.99) and relative standard deviation less than 3% (RSD < 3%). The lowest detection limit was between 0.53 to 2.51 mg/L. Consequently, the determined concentrations of six organic acids (oxalic acid, formic acid, lactic acid, acetic acid, citric acid and propionic acid) were 0.84, 3.69, 51.13, 20.61, 1.76 and 1.06 mg/g respectively. In conclusion, RP-HPLC was a convenient, rapid and accurate method for analysis of organic acids in fermentation-silage of citrus peel wastes.
