Determination of Collagen Content in Livestock and Poultry Ossein by Spectrophotometry
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Abstract:
The paper provided a rapid, simple, accurate and low cost method to determine collagen content in ossein. Collagens from livestock and poultry bone were extracted by acid and thermal processes, and then calculated the ratio of collagen protein and hydroxyproline content in order to obtain collagen conversion factors from different bones. However the results indicated that fewer differences of collagen conversion factors between water-soluble and acid soluble collagen could be seen. According to the actual production, water-soluble collagen was taken as the conversion factor in ossein. The hydroxyproline in ossein was measured by spectrophotometry and then the collagen contents of pig, bovine and chicken ossein were calculated as 9.19%, 7.92% and 9.78%, respectively. The relative standard deviation (RSD) was between 0.01% and 0.03% and coefficient variation (CV) changed from 0.44% to 2.26%. The average recovery rate ranged from 99.36% to 100.44%. In conclusion, the method was appropriate to detect of collagen content in livestock and poultry ossein.
