Detection of Arcobacter butzleri in Animal Derived Food by a PCR Method
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Abstract:
Arcobacter spp. are newly emerging human foodborne pathogens, of which A. butzleri has the highest contamination rate PCR method amplifying 23S rRNA gene was applied to specifictly detect Arcobacter butzleri in this study, and the sensitivity of the method was 103 CFU/mL. Both of the 2 different reference strains of A. butzleri amplified and formed a band with length of 2061 bp, while A. cryaerophilus, A. skirrowii, Campylobacterjejuni and other different strains, 18 strains in total, didn’t produced any band. The results proved the PCR method had high specificity and could be used to identify A. butzleri at the species level. The comparative experimental results showed the PCR method also had advantage over the API CAMPY test kit, as the latter could only be used to identify A. butzleri at the genus level. 55 animal derived food samples containing the strains were enriched in Johnson-Murano broth and then detected by the PCR method, of which 5 samples were detected positive for A. butzleri, with the positive ratio of 9.1%. It suggested that the method was specific, easy to operate and timesaving, which could be used for rapid detection of A. butzleri in animal derived food.
