Simultaneous Analysis of Six Water-soluble Sugars in the Main Stream Smoke of Cigarettes by Ion Chromatography with Pulsed Amperometric Detection
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Abstract:
Arabinose, galactose, glucose, xylose, mannose and fructose are the six major water-soluble monosaccharides in the main stream smoke of cigarette. These sugars were separated and determined by high-performance anion exchange chromatography with pulsed integrated amperometric detection. Soluble sugars of the main stream smoke trapped in a Cambridge filter were extracted with 20 mg/L sodium azide by ultrasonic wave for 45 minutes. Six sugars were effectively separated by using 2.5 mmol/L NaOH as eluent on an anion exchange column -CarboPac PA20. The detection limits were between 2.47~9.82 ?g/L and the linear correlation coefficients were above 0.999 in all cases. The intra-day and inter-day precision were below 5.0% and 6.9%, respectively. The recoveries of three spiked levels ranged from 97.1% to 102.4%. The established method was successfully applied in the detection of cigarette samples of various brands. The total contents of six sugars within the tested samples were 36.17~119.5 ?g/cigarette. Fructose had the highest content with the values between 24.63~74.66 ?g/cigarette. And glucose showed the second highest content, which took up 8.09 to 38.09 ?g/cigarettes. Both fructose and glucose accounted for 90.5~94.7% of the total contents of six sugars.
