Abstract:Picric acid colorimetric method was conducted to measure TMA and TMAO of Litopenaeus vannamei in this research. Variations as the mole ratio of TMA and TMAO, total volatile basic nitrogen (TVB-N), pH value and sensory evaluation were detected to determine the freshness of Litopenaeus vannamei at the store temperature of 0, 5 and 15 ℃. The result showed that TMA, TMAO and pH values grew unsteadily which were hardly to detect the freshness of sample whereas the mole ratio of TMA and TMAO and TVB-N increased steadily with the increasing storage time. Moreover, the mole ratio of TMA and TMAO and TVB-N were significantly correlation (p＜0.01), and all the correlation coefficients were greater than 0.936. Thus the mole ratio of TMA and TMAO was a feasible index for detection of the freshness of Litopenaeus vannamei simply and exactly.
