Inactivation of Microorganisms in Model Fruit Juice System by Dynamic Ultra-high Pressure Combined with Dimethyl Carbonate
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Abstract:
Inactivation of dynamic ultra-high pressure (DUHP) combined with dimethyl carbonate (DMDC) on E. Coli, Saccharomyces cerevisiae and Leuconostoc mesenteroides in model juices were investigated. The results showed that the effect of DUHP on inactivation of three strains in model juices gradually increased as the pressure increased (p<0.05). UHP had the best sterilization effect on E. Coli under the same pressure. But even under the pressure of 100 MPa, the reduction of E. Coli was not more than 1.2 Lg cycles. After 250 mg/L DMDC was added to the model juices, E. Coli and Saccharomyces cerevisiae were completely inactivation after 2 h, while Leuconostoc mesenteroides only decreased 1.7 Lg cycles. No significant reduction was displayed as treatment time increased. DUHP combined with DMDC showed a significantly synergistic effect on the inactivation of Leuconostoc mesenteroides (p<0.05), and its sterilization effect was affected by the sequence of DMDC addition.
