Analysis of the Chemical Constituents of Defensive Substances from Aspongopus chinensis and their Effect on the Activity of LO2 Cells
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Abstract:
The volatile components and their relative contents of the defensive substances in aqueous solution from Aspongopus chinensis, and the effect of aqueous solution on the activity of normal human liver LO2 cells were studied in this research.Warm water immersion of bugs was used to obtain the defensive substances solution, and GC-MS and area normalization was employed to identify the volatile components and calculate their relative contents. MTT and flow cytometry was used to find the effect of the defensive substances solution on liver LO2 cell. The research showed that the solution contained 19 kinds of chemical compounds, and the anti -2- hexenal had the highest relative percentage content 88.54%, then followed by 2-methyl-4-pentene aldehyde (5.16%) and trudecyl (3.43%); IC50 was 3.64 μL which presented dose-dependent manner after the solutions reacted with LO2 cells. The result of flow cytometry showed that, compared with the control group, the percentage of G2/M phase cells from the drug group were reduced obviously and G0/G1 phase cells were increased significantly. The major compounds of Aspongopus chinensis defensive substances solution were enal and alkanes, which could inhibit the proliferation of normal human liver LO2 cells in vitro because of cell cycle arrest.
