The Antioxidant Activities of Walnut Protein and its Enzymolysis Product
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Abstract:
The antioxidant activities of walnut dreg, walnut dreg protein extract, walnut kernel, peeled walnut kernel, defatted walnut kernel, peeled and defatted walnut kernel hydrolysates were researched. The results showed that, the order of reducing power of each hydrolysate was as follows: walnut kernel ? walnut dreg protein extract ? walnut dreg ? defatted walnut kernel ? peeled walnut kernel ? peeled and defatted walnut kernel. When the substrate concentration was 10 mg/mL, the reducing power of walnut kernel hydrolysate was 79.74% of the glutathione. The sequence of ORAC value was as followed: walnut kernel ? defatted walnut kernel ? walnut dreg protein extract ? walnut dreg ? peeled walnut kernel ? peeled and defatted walnut kernel. Walnut kernel hydrolysate had the highest ORAC value 1560.75 μmol Trolox equivalent/g peptide, which was equal to the same weight of the glutathione. All the enzymolysis products could protect PC12 cell damage induced by H2O2, compared with other hydrolysates, the walnut kernel had the best protective effect. When its substrate concentration was 0.10 mg/mL,0.25 mg/mL and 0.50 mg/mL, the cell survival rate reached 72.64%, 90.43% and 84.98% respectively. When the substrate concentration was 1.00 mg/mL, the best protective effect was defatted walnut kernel with the cell survival rate being of 85.11%.
