Establishment of High Performance Liquid Chromatographic Fingerprint of Southern Route Tea
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The chromatographic fingerprint of southern route tea was established by high performance liquid chromatography (HPLC). A suitable chromatographic system was established using a linear gradient elution with acetonitrile and water containing 0.3% formic acid as the mobile phase with a detection wavelength of 280 nm and the flow speed of 1.0 mL/min. According to the average relative retention time, common peaks, and average relative peak area in HPLC were assayed respectively. The HPLC fingerprint was established with good stability, precision and reproducibility. The chromatographic fingerprint was analyzed based on the analysis of HPLC chromatogram of 48 batches of southern route tea samples. It was confirmed that 37 peaks in chromatograms can be selected as characrerixation peaks while the caffeine peak was chosen as the referential peak. The HPLC fingerprints were analyzed by similarity evaluation and cluster analysis to conduct the chemical pattern recognition. The similarity of 48 batches of southern route tea was more than 0.90. Results from a cluster analysis of the relative peak area showed that the 48 batches of southern route tea samples could be divided into about two types.
