Quantitative Analysis of Five Polyphenols in Rhizoma smilacis Glabrae by HPLC
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Abstract:
A HPLC quantitative method for simultaneous determination of 5-O-caffeoylshikimic acid, astilbin, taxifolin, engeletin and trans-resveratrol in Rhizoma Smilacis Glabrae (RSG) was developed. An Agilent Zorbax SB C18 column (250 mm×4.6 mm i.d., 5 μm) was used. The mobile phase consisted of acetonitrile (A) and 0.1% acetic acid aqueous solution (B) with linear gradient program of 16~21% (A) in 0~15 min, 21~40% (A) in 15~40 min. The calibration curve of each analyte was constructed under six concentrations. The correlation coefficients were all >0.998, and the RSD values of tR and the peak area ranged from 0.22 to 0.76% and from 2.67 to 4.64%, respectively. Among 18 RSG samples, astilbin was the most dominant constituent with content ranged from 5.48 to 25.75 mg/g. The total amount of seven flavonols including taxifolin, engeletin, isoengeletin, astilbin and its three isomers (quantified as astilbin) was in the range of 6.72~35.3 mg/g, with average of 18±8.5 mg/g. 5-O-caffeoylshikimic acid was the second dominant component with content ranged from 0.97 to 6.29 mg/g, while the contents of taxifolin and trans-resveratrol were relatively low, and in some samples was even undetected.
