Determination of Ergosterol by HPLC in the Fermentation of Ganoderma lucidum
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Abstract:
The ergosterol content in fermentation of Ganoderma lucidum was determined by HPLC at 282 nm. The results showed that the maximum standard deviation was 0.92% and the linear regression equation y= 28685x-382.61 (R=0.9998) in the range of 20~500 μg. The precision test showed that Ganoderma lucidum extracellular and intracellular ergosterol content of RSD were 0.40% and 1.96%, respectively. The spike recoveries for ergosterol were between 96.82 and 102.50%. The method was feasibility and accurate for determination of ergosterol content in fermentation of Ganoderma lucidum. The ergosterol content of Ganoderma lucidum extracellular and intracellular were 0.07 mg/mL and 0.64mg/g, respectively, after fermentation for 120 h.
