Biosynthesis of ZnO Nanoparticles by Lactobacillus salivarius L3
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Abstract:
In order to obtain the inerratic crystal of ZnO nanoparticles, ZnO nanoparticles was biosynthesized by using Lactobacillus salivarius L3. Lactobacillus salivarius L3 strain was isolated from faeces of healthy weaning piglets weaned at 7~10 d age. Effect of temperature, action time, raw liquor of pH value and the concentration of zinc solution on the synthesis were investigated. The optimum synthesis factors were determined by orthogonal experiment as follows: 0.25 M ZnCl2, pH 7.0, water bath temperature 70 ℃ and reaction time 25 minute. Under the optimum synthesis conditions, the size of the zinc oxide crystal grain were nearby 70 nm. Meanwhile the crystal shape was uniform and had a strong antibacterial capability against E. coli, Salmonella and Staphylococcus aureus. The minimum inhibitory concentration was 0.024 mg/mL to E. coli, 0.029 mg/mL to Salmonella,and 0.016mg/ml to Staphylococcus aureus. Using ZnO nanoparticles instead of normal ZnO into animal feed will effectively enhance the growth performance of weanling pigs.
