Characteristics of Lentinan Polysaccharide Extracted by Ultrasonic Wave and Hot Water
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Abstract:
The extraction of lentinan polysaccharides by Ultrasonic wave used in the first step(62 ℃, 50 min, 640 W) and hot water used in the second step (120 ℃, 40 min) was investigated. The extraction yield reached 15.57±0.83%, being 1.39 times of that of the polysaccharides prepared by traditional water extraction. After the first extraction step, the polysaccharides was further extracted by high-temperature water, giving higher purity of 91.98%, a smaller molecular weight of 3.7×105 Da and viscosity of 1.07 pa?s. A red shift phenomenon was observed when conducting Congo red experiments. The polysaccharides extracted in the first step showed an evident red shift, indicating that the polysaccharides extracted by ultrasound had complete triple helix structure, which were further confirmed by a scanning electron microscope. Infrared spectra showed that the chemical structural of the two polysaccharides had no significant difference.
