Antioxidant Activity of Ethanol Extracts of Loquat Flowers at Different Flowering Phases
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Abstract:
The changes of flavonoids and the total phenolics contents and antioxidant activity in four different stages of loquat flowers (the budding, the bud swelling and white petals forming , the petals spreading and the petals opening stages) were studied using twice reflux extraction (70% ethanol as solvent) for extraction of loquat flower. Results showed that antioxidant materials contents and antioxidant activity of the extracts of flower sampled from the bud stages to the petals opened of loquat flowers exhibited an initial increase and a final decrease. Compared with the samples at other three flowering phases, the flowers samples at the stage of the bud swelling and white petals forming showed the highest flavonoids, the total phenolics and Vc contents, being of 13.39±0.45 mg/g, 76.06±1.18 mg/g and (2.73±0.28)?10-2 mg/g, respectively. In this phase, the flower extracts also showed the highest scavenging ability on?OH free radical, reducing power, total antioxidant capacity and scavenging ability on DPPH free radical, indicating that loquat flower at the phase of bud swelling can be used for further development.