Functional and Conformational Properties of Arachin and Conarachin
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Abstract:
A comparative study was made on functional and conformational properties of arachin and conarachin isolated by ammonium sulfate. Conarachin had the higher solubility than arachin except at near pI (pH 4.5~6.0). And conarachin had better emulision ability index (70~180 m2/g) and foam capacity (36~57%) than arachin (60~130 m2/g and 19~33%). The G’ of conarachin was 5 times higher than that of arachin. Arachin had higher denaturation temperature (104.84 ℃and ΔH value 13.78 J/g) than conarachin (89.47 ℃ and 8.11 J/g) (P<0.05). Most of free SH of arachin was buried in the interior, but located at the surface of conarachin.. Compared with conarachin, arachin had more compacted tertiary conformation and lower surface hydrophobicity.