Oxidation Modification of Clove Anti-low-density-lipoprotein by Bio-assay Guided Method
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Abstract:
Clove, the dried buds of Eugenia caryophylata Thunb, showed the strongest antioxidant activity among 87 kinds of medicinal and edible plants in our previous researches. In this research, the anti-Low-Density-Lipoprotein (LDL) oxidation modification of clove was studied by determining the yield of Malondialdehyde and using bio-assay guided method and copper sulfate induction. The results showed that the appropriate concentrations of copper sulfate and LDL were 3 mmol/L and 4 mg/mL, respectively. The crude extract and different fractions of clove possessed strong anti-LDL oxidation activity. The inhibition efficiency of ethyl acetate fraction was higher (85.29±0.02)% than that extracted by ethyl acetate n-butyl alcohol and water. The inhibition rate of ethyl acetate fraction on LDL oxidation modification presented a good dose-effect relationship. There are eight kinds of ingredients in effective fraction through TLC analysis.
