Isolation, Identification and Phylogenetic Analysis of Culturable Fungi in Luzhou-flavor Daqu
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Abstract:
69 strains of fungi were isolated from Luzhou-flavor Daqu through traditional microbial isolation method．The taxonomy information of these separated fungi were analyzed through morphologic observation, molecular biological identification and phylogenetic analysis. The results showed that most of fungi can be identified to genus, and species of some strains were identified based on molecular identification of ITS region sequences. Aspergillus, Monascus, Rhizomucor, Lichtheimia, Penicillium, Paecilomyces, Saccharomyces cerevisiae, Debaryomyces, Wickerhamomyces anomala, and Sporidiobolus pararoseus were found as the main strains of Luzhou-flavor Daqu. Other groups like Merimbla ingelheimensis，Talaromyces, Cercospora，Cladosporium, Acremonium impicatum, Neurospora etc were also found in Luzhou-flavor Daqu. Among them, Aspergillus spp. was the dominant group, and its stain number ratio and species number ratio were 43.48% and 20.69%, respectively.
