Effect of Astragalus Polysaccharide on Immune Function of Erythrocyte in Tumor Model Mice
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Abstract:
The effect of Astragalus polysaccharide on membrane structure and components of erythrocyte were studied and its effect on erythrocyte immune function in tumor model mice was also studied. S180 mice were made as tumor model mice, and administrated with Astragalus polysaccharide by i.p. for 7 d, and then the erythrocytes in mice venous blood were collected. Band 3 and glycophorin A content were determined by SDS-PAGE. Spectrophotometer with test kit was used to measure phospholipids, cholesterol and sialic acid. DPH dye and fluorescence spectrophotometer were used to determine membrane fluidity. Erythrocytes were labeled by rat anti-mouse CR1 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Flow cytometry was used to determine the number of CR1, and then the DTER and RBC-C3bRR were determined by microscope. The results showed that Astragalus polysaccharide increased phospholipids content by 0.17 mmol/L and decreased cholesterol content by 0.06 mmol/L. In addition, the contents of Band 3, glycophorin A proteins and sialic acid were increased by 2.88%, 7.40% and 0.23 mmol/L, respectively. Erythrocyte membrane fluidity was also improved remarkably. The use of Asparagus polysaccharide also resulted in the increase of CR1 number, DTER and RBC-C3bRR in tumor model mice by 5.9%, 3.13% and 3.26%, respectively. These results indicated that Astragalus polysaccharide can adjust the abnormality of erythrocyte in tumor model mice, improve membrane structure and components, and restore membrane fluidity and function. Asparagus polysaccharide can also improve the erythrocyte immune function by affecting CR1.
