Separation and Identification of Main Pathogen in Hami Melon during Cold Storage
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Abstract:
Pathogenic bacteria which may cause the rot of melon was separated and identified from rotting postharvest Xinjiang Hami melon during cold storage, by using traditional pure culture method, dilution spread plate method and fungal genomic DNA isolation Kit method. The physiological and biochemical properties of the strains, as well as their 26s rRNA gene sequence, were analyzed. Taking NL1 and NL4 as the universal primers, 5 strains of pathogenic bacteria were separated and confirmed as Fusarium, Stachybotrys, Penicillium and Gibberella. The BJ-M and BJ-M1 strains belonged to the genus Fusarium and BJ-M2 strains belonged to Gibberella. BJ-M3 strains belonged to Stachybotry and BJ-Q strains belong to the genus Penicillium. This research may provide theoretical basis for storage period of Hami Melon by investigation of the pathogenic bacteria.
