Development of an Immunoassay for Detection of Naproxen in Health Liquor
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Abstract:
Naproxen,a nonsteroidal anti-inflammatory drug, was frequently reported being adulterated onto antirheumatic health liquors to promote therapeutic effect. This phenomenon poses a serious threat to public health. In order to develop an immunoassay for detection of naproxen, providing an analytical method for rapid screening of illegal products, naproxen was conjugated to BSA and OVA to synthesize artificial antigens using active ester method for preparation of immunogen and testing antigen, respectively. Immunogen was employed to immunize rabbits for raising polyclonal antibodies. An enzyme-linked immunosorbent assay (ELISA) was established based on the antibodies and testing antigen. The IC50 value and LOD of the optimal ELISA were 23.0 ng/mL and 3.1 ng/mL, respectively. The recoveries were 87.3 ~102.1% and the coefficients of variation (CV%) were less than 8.9% when detecting Naproxen in health liquors. All cross-reactivities against 8 associate drugs were less than 0.05%. The ELISA method was in good agreement with LC-UV when detecting Naproxen in 1 positive and 19 negative health liquors from market. The method was suitable for screening Naproxen as illegal additives in health liquors due to its sensitivity and specificity.
