Extraction of Volatile Oil from Tangerine Peel by Supercritical Carbon Dioxide (SC-CO2) and Analysis of Its Chemical Constituents
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Abstract:
Process conditions of extracting volatile oil from tangerine peel were studied by supercritical carbon dioxide (SC-CO2), and chemical constituents of the oil were analyzed by gas chromatography coupled with a mass spectrometer (GC–MS). The effects of extraction temperature, pressure, time, and particle size of tangerine peel on extraction yield of volatile oil from tangerine peel were investigated by single factor experiments. Based on the results of the single factor experiments, orthogonal test of extracting the oil was carried out. Extraction pressure and extraction time as highly significant influencing factors, and extraction temperature as significant influencing factor on extraction yield of the oil were confirmed by analysis of variance (ANOVA). In addition, the optimal conditions were determined by orthogonal test as follows: extraction temperature 35 ℃, extraction pressure 16 MPa, extraction time 120 min, and particle size of tangerine peel 30 mesh. Under the above conditions, extraction yield of volatile oil from tangerine peel was 3.96 % and polymethoxyflavones content of the oil was 33.6 %. GC–MS analysis revealed that the main three constituents of the oil were 9,12-Octadecadienoic acid (Z,Z)-methyl ester (31.90 %), 9-Octadecenoic acid-methyl ester(E) (24.52 %), and 9,12,15-Octadecatrienoic acid-methyl ester (17.53 %).
