Abstract:In this study, a sandy tea product was purchased for the evaluation of in vitro anticancer effect on TCA8113 human tongue squamous carcinoma cells and in vivo anti-metastatic effect．By using MTT assay, apoptosis analysis by DAPI staining and RT-PCR assay, and western blot assay were employed to check the in vitro anticancer effect and in vivo anti-metastatic effect of Sandy tea at different concentration (50, 100 and 200 μg/mL) using animal models. Results showed that 200 μg/mL of sandy tea (81%) had the best inhibitory effect and apoptosis-inducing activity on TCA8113 human tongue squamous carcinoma cells compared to those samples at other concentrations.With increasing the tea concentrations, mRNA expressions of Bax, caspase-3 and caspase-9 was up-regulated, but Bcl-2 expression was down-regulated. BALB/c tumor anti-metastasis test showed that sandy tea inhibited in vivo tumor metastasis induced by colon 26-M3.1 cells in BALB/c mice. These results suggest that sandy tea had potential as an anticancer functional food.
