Abstract:Through oxford cup test and half dilution method for testing antibacterial activity, the preparation progress and antibacterial activity of 10-HDA against Bacillus subtilis were identified and analyzed in this paper. The quantity and bonding of bacteria DNA affected by 10-HDA were analyzed by using atomic force microscope (AFM) and agarose gel electrophoresis of DNA. The results showed that, 10-HDA was a broad-spectrum antimicrobial agent and had obvious inhibition effects on multiple pathogenic bacteria with a concentration-depended mode of inhibitory effect. The minimum inhibitory concentration (MIC) of Bacillus subtilis was 0.62 mg/mL. Agarose gel electrophoresis and AFM analyses revealed that the synthesis of DNA was rapidly decreased due to the close combination with 10-HDA. It was further proved by its impact on PCR of DNA that the synthesis of DNA could be completely impeded when the mixing concentration of 10-HDA was 1.0mg/ml. Therefore, bacteriostatic action was inferred to be caused by the combination between 10-HDA and DNA which inhibited the replication of DNA.
