Comparison of the Typing Methods of ERIC?PCR and Biochemical for Campylobacter jejuni Isolates
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Abstract:
To establish an ERIC?PCR molecular typing method for Campylobacter jejuni genotyping, the subtyping effect of ERIC-PCR and biochemical typing methods were compared. L16 (54) orthogonal text was designed with four factors of Mg2+, dNTPs, primers, TaqDNA polymerase concentration to explore the suitable reaction conditions in a wide range. Then three factors (except the primers), template concentration and annealing temperature were further optimized in a small range for final optimization. The optimized ERIC?PCR method was used to type the 24 C. jejuni isolates. According to the results of biochemical reactions to biochemical type the strains, the ERIC-PCR molecular typing method was compared with biochemical typing method. C. jejuni strains were grouped into 22 types. The ERIC?PCR method exhibited better disciminative results in molecular typing with discrimination index of 0.92. The C. jejuni isolates were classified to 19 biochemical types by biochemical typing method, which showed the diversity of genes in the strains. The ERIC?PCR subtyping method showed the genetic diversity of the strains better than biochemical typing method, which was an efficient method for typing and tracking analyses. It was suitable for genetic diversity analysis of of C. jejuni strains.
