Expression Changes of Proteins in Fermentation Broth of Aspergillus oryzae
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Abstract:
In this paper, biomass accumulation, dynamic expression of proteins in fermentation broth of Aspergillus oryzae were studied when glucose or tween 80 were used as additional carbon sources. When glucose was used as additional carbon source, biomass accumulation of A.oryzae was faster than tween 80 and reached the maximum of 31.97 g/L at 24 h, but accumulation of A.ryzae was slow when tween 80 was used, which reached the maximum of 11.15 g/L at 96 h. Total proteins in fermentation broth was higher with glucose as additional carbon source. Cell-bound proteins reached the maximum value of 5.65 mg/g at 72 h with tween 80 as additional carbon source, while cell-bound proteins reached the maximum of 1.71 mg/g at 48 h with glucose as additional carbon source. Expression of cell-bound proteins detected by SDS-PAGE within 192 h showed that cell-bound proteins increassed at the rapid growth and stationary phages, and reduced at the recession phage.which was in consistent with protein contents measured by the Lowry method.
