In Vitro Antioxidative Activity of Germanium-rich Exopolysaccharides from Ganoderm Applanatum
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Abstract:
Germanium-rich exopolysaccharides (GEPS) was prepared via submerged fermentation of Ganoderm applanatum, and the antoxidative activity of GEPS in vitro was evaluated by determining its capability in eliminating superoxide anion free radical, hydroxyl radical, DPPH?and sodium nitrite. Also, those various antioxidant activities were compared to Vc. Results showed that GEPS reached 1.12 g/L, being of 36.62% higher than those of the control. The organic Germanium reached 0.26 mg/g, being of 116.67 % higher than those of the control when the concentration of GeO2 in culture medium was 150 μg/mL. The organic Germanium could greatly enhance the free scavenging effective to superoxide anion free radical, hydroxyl radical, DPPH?, sodium nitrite and deoxidization capacity of exopolysaccharides, being of 25.52%, 31.83%, 26.95% and 16.38%, respectively, higher than those of the control when GEPS was 2.5 mg/mL. The antoxidative activity of GEPS displayed a dose-response relationship with its concentrations.
