Antibacterial Activity of Pu’er Tea Extracts in Vitro
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Abstract:
The antibacterial activity of the solvent extracts from Pu’er tea in vitro was studied in this paper. Filter paper disc diffusion method and minimal inhibition concentrations (MIC) were employed to determine the antibacterial activities of Pu’er tea extracts by water, 75% and 95% ethanol on S. aureus, E. coli, P. aeruginosa and B. subtili s. The 95% ethanol extracts showed the highest antibacterial effect, especially to S. aureus and E. coli.with the inhibition radius being of 19.58 ± 0.24 mm and 14.58 ± 0.24 mm, respectively. The MIC was 0.63 mg/mL, 0.39 mg/mL. But 95% ethanol extracts showed little inhibitory effect on the growth of B. subtilis. The extracts obtained using different solvent showed different antibacterial effects, amont which the ethyl acetate extracts showed the strongest antibacterial activities, followed with the extracts of n-butyl alcohol, petroleum ether and chloroform. For different bacteria tested, the ethyl acetate extracts showed the strongest effects on the growth of S. aureus with the inhibition zone diameter being of 31.88 ± 0.24 mm and the MIC being of 0.473 mg/mL. The ethyl acetate extract of Pu'er tea could significantly inhibit the growth of gram-positive and gram-negative bacteria. This study provided the references for the development and utilization of antibacterial active ingredients from Pu'er tea.
