Isolation and Identification of Endophytic Fungi Associated with Pavona Lamarck of Xuwen Natural Reserve
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Abstract:
To study the diversity of fungi derived from Pavona lamarck, spread-plate method and two different media were used to cultivate and isolate the strains. The strains was identified from the colony morphology, the individual form of mycelium and spores referring to fungi identification manual. Molecule was identified according to the ITS-rRNA gene sequence features. Based on the result of sequencing, a phylogenetic tree was build for population diversity analysis. 10 symbiotic fungis were isolated from the Pavona lamarck, belonging to 5 generas including 3 Aspergillus spp., 3 Hypocrea spp., 2 Penicillium spp., 1 Cladosporium spp. and 1 Phlebia spp., indicating that Aspergillus and Hypocrea were the dominant fungi. Compared to other corals, the fungis isolated from Pavona lamarck were fewer, but had a high degree of uniformity. There was no report on marine fungis.
