Rapid Determination of Caffeine in Children's Chocolate-containing Foods by Pseudo Reversed Phase Liquid Chromatography
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Abstract:
In this paper, a rapid and sensitive method based on pseudo reversed phase liquid chromatography (pseudo-RPLC) was developed for analysis of caffeine in children's chocolate-containing foods. The samples were analyzed on a polar silica column using an aqueous-rich mobile phase (sodium formate 0.03 mol/L, pH 2.9-acetonitrile, V/V, 95:5). All the samples were tested within 5 min. In view of less organic modifier (only 5%) in mobile phase and shorter analysis time, it was not only economical, but also environment-friendly. In addition, the assay was validated in the concentration range of 0.2~1000 μg/mL. The proposed method was applied to chocolate-containing foods. Recoveries at the concentration of 20~60 μg/mL ranged from 96.5% to 104.3% with RSD< 4.8%. The present pseudo-RPLC technique proved to be a good alternative of reversed-phase liquid chromatography for the analysis of caffeine in popular chocolate-containing products with the advantages of fast-speed, low-cost and selectivity. Moreover, the retention mechanism of caffeine was discussed.
