Determination of Benzo[α]pyrene in Camellia Oil by Gas Chromatography Tandem Mass Spectrometry
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Abstract:
A method was developed for the determining of benzo[α]pyrene in camellia oil by gas chromatography by tandem mass spectrometry (GC-MS/MS) with liquid-liquid extraction. After spiked with the isotopic labeled benzo[a]pyrene - d12, the sample was extracted twice with acetonitrile, and then defatted by n-hexane to obtain an extract suitable for analysis using GC-MS/MS. The targeted compound was separated by a DB 5-MS column detected by GC-MS/MS system under the mode of multiple reaction monitoring (MRM). The internal standard was used for quantitative analysis. Under the optional conditions, the linear range was between 0.50 μg/L and 100 μg/L with the linear correlation coefficients of 0.9994. The detection limit of the method (LOD) was 0.25 μg/kg and the quantitation limit of the method (LOQ) was 0.5 μg/kg. The mean recoveries for camellia oil at three spiked concentrations levels of 0.50, 1.00, 5.00 μg/kg were ranged from 92.51% to 103.70% , respectively, with relative standard deviations (RSDs, n=6) 3.84~5.80%. The inter- recoveries were ranged from 94.71% - 110.83% and the precision was ranged from 5.22% to 8.92% (n=5). This method was suitable for the identification and quantification of benzo[α]pyrene in camellia oil due to its simplicity, accuracy and high sensitivity.
