Development of an Immunoassay Method for Detection of Sulindac in Health Liquor
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Abstract:
lindac is a nonsteroidal anti-inflammatory drug. Recently, it has been found that undeclared sulindac was frequently added into antirheumatic health liquors to promote therapeutic effect, which poses a serious threat to public health. In order to develop an immunoassay for detection of sulindac, providing an analytical method for rapid screening of illegal products, sulindac was conjugated to BSA and OVA to synthesize artificial antigens using active-ester method for preparation of immunogen and testing antigen, respectively. Immunogen was employed to immunize rabbits for raising polyclonal antibodies. An enzyme-linked immunosorbent assay (ELISA) was established based on the antibodies and testing antigen. The IC50 value and LOD of the optimal ELISA were 18.1 ng/L and 3.5 ng/mL, respectively. The recoveries were 76~16% and the coefficients of variation (CV%) were less than 7.6% when detected sulindac in health liquors. All cross-reactivities against 8 associate drugs were less than 0.05%. The ELISA was in good agreement with LC-UV when detecting sulindac in 2 positive and 28 negative health liquors from market. The method was suitable for screening sulindac as illegal additives in health liquors due to its sensitivity and specificity.
