Determination of Proanthocyanidins in Mangosteen by 4-(Dimethylamino)cinnamaldehyde Assay
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Abstract:
4-(Dimethylamino)cinnamaldehyde (DMAC) was introduced as a chromogenic agent to determine proanthocyanidins by spectrophotometry, and the content of proanthocyanidins in rind, calyx lobes and aril of mangosteen were detected by DMAC assay. The condensation compound of DMAC and catechin was stable and can be measured in 15~35 min after reaction, while DMAC solution is suggested to be used fresh daily. Vanillin assay was also conducted in determining mangosteen proanthocyanidins, which showed a higher content but less sensitivity compared with DMAC assay. The standard curve was y = 0.0937x - 0.0029（R2 = 0.9993）in DMAC assay. Proanthocyanidins content in rind, calyx lobes and aril of fresh mangosteen were 4.23±0.24 mg/g, 0.85±0.02 mg/g and 0.53±0.03 mg/g, respectively, using catechin as standard.
