Purification of Alkaloids of Leaves of Nelumbonucifera Gaertn by Macroporous Adsorption Resin
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Abstract:
For purification total alkaloids of Nelumbonucifera leaves, capability of adsorption and desorption of three kinds of macroporous adsorption resin such as AB-8, D101 and HP-20 were explored. Then the effects of adsorption parameters, such as flow rate of adsorption, pH value, maximum loading amount of the extract solution; concentration, pH value of eluant and flow rate of desorption, on the nuciferine content were investigated by HPLC. The results showed that D101 was the most efficient Resin. The optimum condition were 2BV/h of loading rate, 10 of pH value, 6 BV of maximum loading amount of the extract solution, flow rate of desorption 2BV/h and pH 3 of the eluent (70% aqueous ethanol solution). Under such conditions, the ratio of nuciferine to the purified extract of Nelumbonucifera leaves was up to 2.26%. This research provided references for comprehensive development and utilization of the total Alkaloids of Leaves of Nelumbonucifera Gaertn.
