Screening of Xylitol-producing Yeast Strains from Soil and Optimization of the Fermentation Conditions
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Abstract:
A yeast strain Y-9 was isolated from soil samples, which could tolerate high concentrations of xylose. HPLC and infrared scanning analysis showed that the main product of the strain Y-9 was xylitol. To improve the xylitol production and conversion rate of strain Y-9, the medium compositions and cultural conditions were optimized by means of single factor experiment and orthogonal tes. Under the optimal cultural conditions (initial xylose concentration 200 g/L, yeast extract 3 g/L, ammonium sulfate 2 g/L, corn steep liquor 10 mL/L, magnesium sulphate 0.2 g/L, initial pH 6, rotation speed 180 r/min and inoculum size 4%), The yield and producing rate of xylitol reached 160 g/L and 1.67 g/L?h, respectively. The conversion rate of xylose reached 80%.
