Abstract:The anti-cancer and anti-inflammatory functions of active components from Stevia Rebaudiana, Taraxacum Officinale and Chrysanthemum Indicum L. were investigated in this article. The components extracted with four solvents (water, ethanol/ethyl acetate, methanol and n-hexane) were separated and purified by silica gel column chromatography and TLC. Murine hepatoma cells (Hepa 1c1c7) and mouse macrophage cells (Raw 264.7) were used to determine quinone reductase activity and inhibition of nitric oxide. Results showed that the strongest activity for all three species was achieved by the ethanol/ethyl acetate extracting; the minimal concentrations to increases double QR specific activity were 0.26~1.59 μg/mL, 0.45~3.73 μg/mL and 0.60~0.92 μg/mL, respectively, for Stevia Rebaudiana, Taraxacum Officinale and Chrysanthemum Indicum L.. The minimal concentrations of Stevia Rebaudiana and Taraxacum Officinale to achieve 50% inhibition of nitric oxide were 14.02~19.04 μg/mL and 48.90~6.05 μg/mL, respectively. The results provided preliminary basis for identification of anti-cancer and anti-inflammatory ingredients and mechanical investigations from the compositae.
