Purification and Antioxidant Activity of Sugarcane Pomace Polyphenols
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Abstract:
Purification technology of polyphenols from sugarcane pomace was studied by static absorption and desorption of macroporous resin, and antioxidative activity of sugarcane polyphenols was evaluated by DPPH free radical and pyrogallol autoxidation. Results indicated that macroporous resin NKA-9 was the best material for purificating polyphenols in sugarcane pomace. The optimum absorption conditions of macroporous resin NKA-9 were concentration of feed 2.5 mg/mL and feeding rate 1.0 mL/min. The optimum desorption conditions were elute-ethanol concentration 60%, elution rate 1.0 mL/min and elute volume 10 BV. Under conditions in which the yield of sugarcane polyphenols was 72.3% and purity was 39.2%. The sugarcane polyphenols showed high scavenging activities on superoxide anion radicals and DPPH radical, and the scavenging activity of polyphenols increased with increasing polyphenol concentration. Sugarcane polyphenols showed lower scavenging activity on DPPH radical but high scavenging activity on superoxide anion radicals than vitamin C. When sugarcane polyphenols concentration was 3.0 mg/mL, its scavenging activity on superoxide anion reached the highest, being of 82.3%. Further increasing sugarcane polyphenol concentration to 5 mg/mL, however, led to the decrease of the scavenging activity on DPPH (80.43%).
