Accumulation and Elimination of Prometryn in Apostichopus japonicus
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Abstract:
Accumulation of Prometryn in Apostichopus japonicus under laboratory conditions was studied using ultra performance liquid chromatogram tandem mass spectrometry. Apostichopus japonicus was divided randomly, and then exposed to water containing 1.00 μg/L, 10.00 μg/L and 200.00 μg/L Prometryn at 15.0±1.0 ℃. After exposure, apostichopus japonicus was placed into clean water for the subsequent elimination experiment. The accumulation amount increased with the increase of Prometryn concentration, presenting significantly positive correlation. The highest concentrations (Cmax) of enriched Prometryn for the three concentrations were 4.70 μg/kg, 43.80 μg/kg and 674.00 μg/kg when placed for 1 d, 3 d and 4 d, and the biggest bioconcentration factors (BCF) were 4.70, 4.38 and 3.37. BCF decreased with the increase of the Prometryn concentration, presenting significantly inverse correlation. When Apostichopus japonicus was bred in clean water, Prometryn accumulated was eliminated rapidly. Prometryn was no detected at 2 d. Dtermination performed for 14 times from 7 h to 46 d, showed 23.70±3.07 μg/kg of Prometryn in Apostichopus japonicus. The accumulation and elimination curves of Prometryn in Apostichopus japonicus provided useful information for decontamination of aquatic organisms that have Prometryn contamination.
