Structural Analysis of Polysaccharides from Pholiota nameko
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Abstract:
A new polysaccharide PNP was isolated and purified from Pholiota nameko and its components and structure were studied. The PNP relative molecular mass and distribution were analyzed by high-performance gel permeation chromatography, the PNP main chain structure were determined by infrared spectra (IR), gas chromatography (GC), partial acid hydrolysis, periodate oxidation, Smith degradation, methylation and GC-MS, 1HNMR, amino acids and β-elimination reaction, and polysaccharide solution action was studied by Congo red and iodine-potassium experiment. The result indicated that the weight-average relative molecular mass of PNP was 20199 Da; PNP was composed of Xyl, Man, Glu and Gal with a molar ration of 1.5: 3.36: 14.2:1; through periodate oxidation and Smith degradation, the branches of PNP were composed of (1→2)-linked, (1→3)-linked and (1→6)-linked with a molar ration of 1.42: 5.06:1, and had no (1→4)-linked; PNP possessed typical infrared absorption of polysaccharides and sugar chain was mainly β-configuration pyranoside and a kind of contained protein glycoconjugate; PNP had triple helical structure containing a long side chain and branches.
